chemi-doc luminescence visualization scanner Search Results


molecular imager chemi doc xrs scanner  (Bio-Rad)
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chemi doc mp scanner  (Bio-Rad)
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chemi doc mp imaging system  (Bio-Rad)
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streptavidin  (Jackson Immuno)
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( A ) A schematic diagram illustratinghow CsFiND proteins biotinylate NVJ-localized proteins. ( B ) Schematic representation of the CsFiND constructs used in this study. ( C ) Yeast cells expressing CsFiND proteins and Nvj1-mCherry were observed by confocal fluorescence microscopy. Scale Bar, 5 µm. ( D ) The biotinylated proteins were purified from membrane fractions isolated from wild-type cells with or without the expression of CsFiND proteins or TurboID-HA and subjected to <t>streptavidin</t> blotting and immunoblotting using the indicated antibodies.
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odyssey fc  (LI-COR)
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LI-COR odyssey fc
( A ) A schematic diagram illustratinghow CsFiND proteins biotinylate NVJ-localized proteins. ( B ) Schematic representation of the CsFiND constructs used in this study. ( C ) Yeast cells expressing CsFiND proteins and Nvj1-mCherry were observed by confocal fluorescence microscopy. Scale Bar, 5 µm. ( D ) The biotinylated proteins were purified from membrane fractions isolated from wild-type cells with or without the expression of CsFiND proteins or TurboID-HA and subjected to <t>streptavidin</t> blotting and immunoblotting using the indicated antibodies.
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chemidoc scanner  (Bio-Rad)
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Bio-Rad chemidoc scanner
( A ) A schematic diagram illustratinghow CsFiND proteins biotinylate NVJ-localized proteins. ( B ) Schematic representation of the CsFiND constructs used in this study. ( C ) Yeast cells expressing CsFiND proteins and Nvj1-mCherry were observed by confocal fluorescence microscopy. Scale Bar, 5 µm. ( D ) The biotinylated proteins were purified from membrane fractions isolated from wild-type cells with or without the expression of CsFiND proteins or TurboID-HA and subjected to <t>streptavidin</t> blotting and immunoblotting using the indicated antibodies.
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odyssey clx apparatus  (LI-COR)
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LI-COR odyssey clx apparatus
( A ) A schematic diagram illustratinghow CsFiND proteins biotinylate NVJ-localized proteins. ( B ) Schematic representation of the CsFiND constructs used in this study. ( C ) Yeast cells expressing CsFiND proteins and Nvj1-mCherry were observed by confocal fluorescence microscopy. Scale Bar, 5 µm. ( D ) The biotinylated proteins were purified from membrane fractions isolated from wild-type cells with or without the expression of CsFiND proteins or TurboID-HA and subjected to <t>streptavidin</t> blotting and immunoblotting using the indicated antibodies.
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adjustable pipettes  (Eppendorf AG)
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( A ) A schematic diagram illustratinghow CsFiND proteins biotinylate NVJ-localized proteins. ( B ) Schematic representation of the CsFiND constructs used in this study. ( C ) Yeast cells expressing CsFiND proteins and Nvj1-mCherry were observed by confocal fluorescence microscopy. Scale Bar, 5 µm. ( D ) The biotinylated proteins were purified from membrane fractions isolated from wild-type cells with or without the expression of CsFiND proteins or TurboID-HA and subjected to <t>streptavidin</t> blotting and immunoblotting using the indicated antibodies.
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cy5 affinipure goat anti rabbit igg h l  (Jackson Immuno)
96
Jackson Immuno cy5 affinipure goat anti rabbit igg h l
( A ) A schematic diagram illustratinghow CsFiND proteins biotinylate NVJ-localized proteins. ( B ) Schematic representation of the CsFiND constructs used in this study. ( C ) Yeast cells expressing CsFiND proteins and Nvj1-mCherry were observed by confocal fluorescence microscopy. Scale Bar, 5 µm. ( D ) The biotinylated proteins were purified from membrane fractions isolated from wild-type cells with or without the expression of CsFiND proteins or TurboID-HA and subjected to <t>streptavidin</t> blotting and immunoblotting using the indicated antibodies.
Cy5 Affinipure Goat Anti Rabbit Igg H L, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) A schematic diagram illustratinghow CsFiND proteins biotinylate NVJ-localized proteins. ( B ) Schematic representation of the CsFiND constructs used in this study. ( C ) Yeast cells expressing CsFiND proteins and Nvj1-mCherry were observed by confocal fluorescence microscopy. Scale Bar, 5 µm. ( D ) The biotinylated proteins were purified from membrane fractions isolated from wild-type cells with or without the expression of CsFiND proteins or TurboID-HA and subjected to streptavidin blotting and immunoblotting using the indicated antibodies.

Journal: bioRxiv

Article Title: Glucose Starvation Sensing through Membrane Remodeling at the Nucleus-Vacuole Junction Coordinates Ergosterol Biosynthesis

doi: 10.1101/2025.05.29.656913

Figure Lengend Snippet: ( A ) A schematic diagram illustratinghow CsFiND proteins biotinylate NVJ-localized proteins. ( B ) Schematic representation of the CsFiND constructs used in this study. ( C ) Yeast cells expressing CsFiND proteins and Nvj1-mCherry were observed by confocal fluorescence microscopy. Scale Bar, 5 µm. ( D ) The biotinylated proteins were purified from membrane fractions isolated from wild-type cells with or without the expression of CsFiND proteins or TurboID-HA and subjected to streptavidin blotting and immunoblotting using the indicated antibodies.

Article Snippet: The transferred proteins were detected by fluorophore-conjugated to secondary antibodies or streptavidin such as Cy5 AffiniPure Goat Anti-Rabbit IgG (H+L) (Jackson ImmunoResearch Laboratories), Streptavidin, (Cy5), (Thermo Fisher Scientific) or goat anti-Rabbit IgG (H+L) cross-adsorbed secondary antibody conjugated to HRP (Thermo Fisher Scientific) and analyzed with Amersham Typhoon scanner (Cytiva) or Chemi Doc Touch (Bio-Rad).

Techniques: Construct, Expressing, Fluorescence, Microscopy, Purification, Membrane, Isolation, Western Blot